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KMID : 1161420120150020135
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Journal of Medicinal Food
2012 Volume.15 No. 2 p.135 ~ p.144
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Molecular Characteristics and Immunomodulatory Activities of Water-Soluble Sulfated Polysaccharides from Ulva pertusa
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Tabarsa Mehdi
Han Jung-H.
Kim Chul-Young
You Sang-Guan
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Abstract
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Sulfated polysaccharides isolated from Ulva pertusa and fractionated using anion-exchange chromatography were investigated to determine their molecular characteristics and bioactivities. The crude and fractionated polysaccharides (F1, F2, and F3) were mainly composed of carbohydrates (59.9?65.9%), sulfates (11.6?15.3%), and uronic acid (7.30?16.4%) with small amounts of proteins (1.40?4.80%). Rhamnose (62.5?80.7%) was the major monosaccharide unit of these polysaccharides, with different levels of glucose (13.5?27.4%) and xylose (2.74?11.5%). The polysaccharides contained one or two major subfractions with weight-average molecular mass ranging from 51.1×103 to 1,690×103 g/mol. The relatively low in vitro anticancer activity of the polysaccharides (22.3?42.4%) suggested that they had little cytotoxicity against the cancer cell line used (AGS). On the other hand, the polysaccharides significantly stimulated Raw 264.7 cells, inducing considerable amounts of nitric oxide and various cytokines production, which suggested that they could be strong immunostimulators.
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KEYWORD
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anticancer, cytokines, immunostimulation, nitric oxide, sulfated polysaccharide, Ulva pertusa
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